Current Synthetic and Systems Biology
Open Access
ISSN: 2332-0737
ISSN: 2332-0737
Department of Biology, University of Saskatchewan, Saskatoon, Canada
Review Article
A Review of Cryopreservation of Cells by Ice Recrystallization Inhibitors (IRIs)
Author(s): Aqsa Sattar, Hafsa Ijaz, Iqra Azam*, Mamoona Idrees and Minahil Azam
Cryopreservation is a process that preserves structurally intact live cells and tissues by freezing them at tremendously
low temperatures for prolonged periods. Ice recrystallization is the major restraint in the cryopreservation of cells,
resulting in lethal cryoinjury. Ice Recrystallization Inhibitors (IRIs), low molecular weight cryo-protectants, restrict
the ice growth and alleviate cell injury during the process of freezing. They may possess the ability to enter the cells
and alter their cryo-biological response to freezing and influence intracellular ice recrystallization as well. In the
presence of abridged quantities of all CPAs including glycerol, ice recrystallization inhibitors can shield the
cells against cryoinjury during the recrystallization process and can also be used as effective cryo-additives to
freeze cells significantly Red Blood Cells (RBCs).. View More»
DOI:
10.35248/2332-0737.23.11.031