Endocrinology & Metabolic Syndrome
Open Access
ISSN: 2161-1017
ISSN: 2161-1017
Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan
Research Article
Effects of Somatostatin Analogs on Catecholamine Biosynthesis Regulated by Corticosteroids and Bone Morphogenetic Proteins in Rat Pheochromocytoma Cells
Author(s): Takahiro Nada, Motoshi Komatsubara, Nahoko Iwata, Yasuhiro Nakano and Fumio Otsuka*
Regulatory roles of SSAs in catecholamine synthesis have not been elucidated. To clarify the actions of SSAs on
catecholamine biosynthesis, we investigated the mutual interactions among SSAs including octreotide and pasireotide,
steroids and BMPs using rat pheochromocytoma PC12 cells. Treatment with octreotide and pasireotide (10 nM to
10 μM) had no significant effect on mRNA levels of Th, DOPA decarboxylase and dopamine-β-hydroxylase in
PC12 cells. Regarding the interaction with steroids, treatments with SSAs also had no effect on dexamethasone- or
aldosterone-induced Th mRNA expression, while pasireotide reduced mRNA expression of the GR. As for the
interaction with BMP-4, which can suppress Th mRNA expression by PC12 cells, SSAs did not affect Th expression
reduced by BMP-4 and Id1 or Smad1/5/9 activation induced by BMP-4. However, BMP-4 treatmen.. View More»
DOI:
10.35248/2161-1017.20.9.307