Applied Microbiology: Open Access
Open Access
ISSN: 2471-9315
+44 1300 500008
ISSN: 2471-9315
+44 1300 500008
Department of Microbiology, School of Life Sciences, Modibbo Adama University of Technology, PMB 2076 Yola, Nigeria
Research Article
Production, Purification and Characterization of Polygalacturonase from Aspergillus flavus Grown on Orange Peel
Author(s): Doughari JH* and Onyebarachi GC
Polygalacturonase is a pectinolytic enzyme that catalyzes the hydrolytic cleavage of the polygalacturonic backbone linkage chain. In this study, polygalacturonase was produced from Aspergillus flavus isolated from an orange waste dump site. Production was carried out in solid state fermentation. Optimum production of polygalacturonase was found at incubation period of 96 h, pH 4.5 and at 35°C using ammonium sulphate, orange peel as best nitrogen and carbon sources, respectively. The enzyme was precipitated with 60% ethanol resulting in 3.54 fold purification, and purification of the enzyme with Sephadex G-75 resulted in 9.93 fold. The purified enzyme showed maximum activity in the presence of polygalacturonic acid at 35°C and pH 4.5, while for sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis, molecular weight obtained was 66 KDa. The K.. View More»