Reproductive System & Sexual Disorders: Current Research
Open Access
ISSN: 2161-038X
ISSN: 2161-038X
Department of Life Sciences, University of Inner Mongolia Agricultural, Hohhot, China
Research Article
Differentiation of Human Umbilical Cord-derived Mesenchymal Stem Cells to Melanocyte, Osteogenic and Neural Cells In Vitro
Author(s): Chunxiao Zhu*, Yuan Guo, Le Liu, Shichao Wang, Junwei Cao, Chunxia Liu, Huanmin Zhou and Yanru Zhang
This study aimed to isolated and culture human Umbilial cord Mesenchymal Stem Cells (hUMSCs) in vitro by tissue block attachment to investigate their biological characteristics. The hUMSCs were cultured to passage 3, following which the induction experiments were performed in vitro and induced to melanocytes, neurons, and osteoblasts. The Reverse Transcription-Polymerase Chain Reaction (RT-PCR) results revealed that the expression of Nanog Homeobox (NANOG), Pou Class 5 Homeobox 1 (OCT4), 5'-nucleotidase ecto (CD73), CD44 molecule (CD44) in umbilical cord-derived mesenchymal stem cells were positive and population doubling time was 24.7 hrs. The differentiation properties of neurogenesis and osteogenic cells were confirmed by histological staining using toluidine blue and Alizarin red. Melanocytes were detected by RT-PCR, quantitative PCR (qPCR), and immunofluorescence staining. Af.. View More»
DOI:
10.35248/2161-038X.23.12.382