Journal of Antivirals & Antiretrovirals
Open Access
ISSN: 1948-5964
ISSN: 1948-5964
Viral Hepatitis Laboratory, Institut Pasteur de Bangui, Bangui, Central African Republic
Research Article
In silico Analyses of Hepatitis B Virus Genotype E Strains from Treatment Naive Central African Patients Reveal Important Mutations in the Complete Genome
Author(s): Giscard Wilfried Koyaweda, Rosaline Macharia, Juliette Rose Ongus, Eunice Machuka, Roger Pelle and Narcisse Patrice Komas*
Background: Hepatitis B Virus (HBV) remains a serious health problem despite the prevention and treatment measures currently implemented. There is little data on the molecular characterization of the strains circulating in the Central African Republic (CAR). Here, we sequenced the full-length genome of HBV isolated from CAR patients.
Methodology: The serum samples were collected at the Institut Pasteur de Bangui. The full-length viral genome was isolated and sequenced using the Sanger technique with four overlapping primers. Sequences were analyzed in silico for mutations and drug resistance using bioinformatics tools.
Results: Four full-length HBV genomes were successfully sequences. All four isolates belonged to genotype E and contained an rtI90L mutation in the Reverse Transcriptase (RT) functional doma.. View More»
DOI:
10.35248/1948-5964-22.14.235