Andrology-Open Access
Open Access
ISSN: 2167-0250
ISSN: 2167-0250
Department of Chemical Sciences, Istituto Nazionale Biostrutture Biosistemi-Consorzio Interuniversitario- Rome, Roma, Italy
Research Article
Quantitative Analysis of Gonadotropins and Thyrotropin in Sera Samples by LC-MRM Mass Spectrometry
Author(s): Anna Illiano, Chiara Melchiorre*, Carlo Cioci, Gabriella Pinto, Francesca Di Rella, Alessandro Conforti, Luigi Carbone and Angela Amoresano
Gonadotropins, as follicle-stimulating hormone (FSH) and luteinizing hormone (LH), belong to a family of
glycoprotein hormones along with human placental chorionic gonadotropin (hCG), and thyroid-stimulating
hormone (TSH). LH and FSH are key regulators of reproduction, and they act in an endocrine manner to regulate
steroidogenesis and gametogenesis in the ovary and testis. Nowadays, highly purified and recombinant formulations
of the gonadotropins are commonly used in the treatment of hypogonadism and infertility. Therefore, the accurate
measurement and characterization of serum gonadotropins is essential to monitor the hormonal response of patient
treatment, but their absolute quantification is a great challenge due to their extensive heterogeneity, and their
very low concentrations in serum. The reference method to quantify circulating gonadotropins, the enzyme.. View More»
DOI:
10.35248/2167-0250.22.S1.002