Journal of Medical Diagnostic Methods
Open Access
ISSN: 2168-9784
+44 1300 500008
ISSN: 2168-9784
+44 1300 500008
Windhagen H
Germany
Research Article
A New Quantification Method of Bacterial Adherence on Implant Surfaces in an Implant Associated Infection Rat Model
Author(s): Schröder ML, Angrisani N, Hegermann J, Windhagen H, Calließ T and Reifenrath JSchröder ML, Angrisani N, Hegermann J, Windhagen H, Calließ T and Reifenrath J
Objective: Implant infections in elective orthopedic surgery are still a clinically relevant problem with devastating consequences for the patient. Thereof results a great need for new strategies to prevent implant infections. Functionalization of implant surfaces to reduce the microbial adherence show great potential in vitro, but have to be tested in suitable models in vivo. Proper evaluation methods of the bacterial load on the implant surface are important for their evaluation. Up to now, the simultaneous assessment of the quantity and morphology of the bacterial infection in vivo was not performed. Methods: Cubic Ti90/Al6/V4-rods were inserted in the tibia of Lewis rats and infected with Staphylococcus aureus strain 36/07 in different concentrations. After 21 days, explanted implants were stained for livi.. View More»
DOI:
10.4172/2168-9784.1000271