Journal of Women's Health Care
Open Access
ISSN: 2167-0420
+44-7360-538437
ISSN: 2167-0420
+44-7360-538437
Neelam Dhiman
Scientific Director, Molecular Infectious Diseases, At The Convergence Center,
2501 South State Highway 121, Suite 1100, Lewisville, TX 75067
Tanzania
Research Article
Diagnostic Evaluation of a Multiplex Quantitative Real-Time PCR Assay for
Bacterial Vaginosis
Author(s): Neelam Dhiman, Charles Yourshaw, Mastan Rao Chintalapudi, Cochanna Turner and Eric MurphyNeelam Dhiman, Charles Yourshaw, Mastan Rao Chintalapudi, Cochanna Turner and Eric Murphy
Background: Quantitative multiplex PCR assay for Bacterial Vaginosis (BV) based on the detection of the predominant contributory targets was evaluated against the conventional Nugent Score that is laborious and subjective due to morphological assessment bias of BV-associated bacteria.
Methods: 125 dual vaginal specimens were collected from patients aged ≥18 years at the time of presentation at the provider office to perform real time PCR and Nugent Testing. PCR assessment of BV was performed by quantitation of DNA amounts of Gardnerella vaginalis, Atopobium vaginae, Lactobacillus spp., and total amount of bacterial DNA using a multiplex RT-PCR kit (ATRiDA, Netherlands). Discordant results were resolved by the Amsel criteria or ancillary testing such as BD Affirm, when available.
Results: Nugent score classified 3.. View More»
DOI:
10.4172/2167-0420.1000293