Journal of Research and Development
Open Access
ISSN: 2311-3278
+44-20-4587-4809
ISSN: 2311-3278
+44-20-4587-4809
Mohammadreza Mohammadabadi
Kerman
West Indies
Research Article
Using Inter Simple Sequence Repeat Multi-Loci Markers for Studying Genetic Diversity in Kermani Sheep
Author(s): Mohammadreza Mohammadabadi, Ebrahim Esfandyarpoor and Ali MousapourMohammadreza Mohammadabadi, Ebrahim Esfandyarpoor and Ali Mousapour
The Kermani sheep has adapted to a tropical region and is an important meat producing animal in Iran and its economic efficiency is mainly dependent on its growth and reproduction ability. The ability of a population to respond adaptively to environmental changes depends on its level of genetic variability or diversity. Thus, genetic diversity in indigenous breeds is a major concern considering the necessity of preserving what may be a precious and irreplaceable richness, regarding new productive demands. Inter simple sequence repeat (ISSR) is the genome region between microsatellite loci. The aim of this study was to evaluate the diversity of Kermani sheep using ISSR markers. DNA was extracted from 100 blood samples of five populations using optimized and modified salting out method. Polymerase chain reaction (PCR) was performed using two ISSR primers (GA)9C and (AG)9C. The amplified.. View More»