Journal of Medical Diagnostic Methods

Journal of Medical Diagnostic Methods
Open Access

ISSN: 2168-9784

Junko Takahashi

Junko Takahashi
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology Tsukuba,
1-1- 1 Higashi, Tsukuba, Ibaraki 305-8563

  • Research Article
    Evaluation for Integrity of Extracted RNA by Reference Material of RNA
    Author(s): Junko Takahashi, Akiko Takatsu and Hitoshi IwahashiJunko Takahashi, Akiko Takatsu and Hitoshi Iwahashi

    Physiological information that identified from RNA expression level in tissues or organs give us benefit in clinical diagnostics. After extracting RNA from blood, tissue, or organs, diagnostics were carried out by using technique for semi-quantitative estimation of specific mRNA, such as real-time PCR or DNA microarrays. Validation of RNA quality need to quantitative estimation of gene expression at the moment of sampling, because using degraded RNA will lead to incorrect results. In this study, we conducted a series of experiment to evaluate RNA integrity using the reference materials of RNA length of 1000 base pairs (refRNA1000), and compared with RIN. There are significant correlation between RIN and the amount of refRNA1000, in varying state of degradation. There are also significant correlations between the amount of refRNA1000 and endogenous gene such as Actb and Rps18 in varyin.. Read More»
    DOI: 10.4172/2168-9784.1000128

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