Journal of Applied Pharmacy
Open Access
ISSN: 1920-4159
ISSN: 1920-4159
Jian Chen
China
Research Article
Two Novel Curcumin Analogues Induced Reactive Oxygen Species Generation and Mitochondrial-Related Apoptosis in Human Breast Cancer MCF - 7 Cells
Author(s): Shuyue Luo, Qingyong Li, Jian Chen and Wengchao WangShuyue Luo, Qingyong Li, Jian Chen and Wengchao Wang
Objective: Curcumin has been shown to have significant protective effects against cancer and induction of apoptosis is a crucial strategy for cancer therapy, so we have now evaluated the mechanisms involved in two novel asymmetric curcumin analogues induced cell death in MCF - 7 cells.
Methods: The cytotoxicity of two curcumin analogues towards tumor cells was investigated by MTT assays. The morphological analysis using a laser scanning confocal microscope. Futher cell cycle analysis, reactive oxygen species (ROS), mitochondrial transmembrane potentials (Δφm), intracellular Ca2+ levels analysis and apoptosis assays via a flow cytometry (FCM). We used western blot assays to determine the expressions of apoptosis-related factors and p38MAPK at protein level.
Results: MCF - 7 cells showed a significant loss .. View More»
DOI:
10.4172/1920-4159.1000215