Molecular Typing using PCR-RFLP Reveals Diversity of Environmental Mycobacteria Agent of Buruli Ulcer in Lvory Coast, Cote d’Ivoire (West Africa).
Author(s): Quinet Gregoire, Kakou Ngazoa E Solange, Aka Nguetta, Vakou Sabine, Coulibaly Ngolo David, Kouakou Helene, Sylla Aboubacar, Faye-Kette Hortense, Aoussi Serge and Dosso MireilleQuinet Gregoire, Kakou Ngazoa E Solange, Aka Nguetta, Vakou Sabine, Coulibaly Ngolo David, Kouakou Helene, Sylla Aboubacar, Faye-Kette Hortense, Aoussi Serge and Dosso Mireille
Buruli ulcer is a neglected skin disease caused by Mycobacterium ulcerans (MU), and affects 1000 people each year worldwide and particularly in African countries. Eradication of Buruli ulcer is difficult because of the lack of early diagnostic in rural endemic regions, and the unknown of the disease in national health care system. In the rural wetlands and marshes in Central and West Africa, children are most affected. MU belongs to environmental Mycolactone Producing Mycobacteria (MPMs) and presents high genetic diversity of the circulating strains. Diagnoses were applied by culture and genome detection by Polymerase Chain Reaction (PCR). The PCR became a gold method to confirm clinical and environmental samples for MU. The MIRU-VNTR method and the Restriction Fragment Length Polymorphism (RFLP) combined with MIRU-VNTR markers were used to discriminate Mycobacteria from different sou.. View More»