Internal Medicine: Open Access
Open Access
ISSN: 2165-8048
+44 1300 500008
ISSN: 2165-8048
+44 1300 500008
Cheng Lan
China
Research Article
Co-regulated Intestinal c-kit by Interleukin-17 and Interleukin-10 on Post-infectious Irritable Bowel Syndrome in Mice
Author(s): Bo Yang, Xuesong Bai, Yijun Yu, Honglan Liu, Cheng Lan, Dan Liu and Xuchun ZhouBo Yang, Xuesong Bai, Yijun Yu, Honglan Liu, Cheng Lan, Dan Liu and Xuchun Zhou
Purpose
The aim of this study was to investigate the probable immune mechanisms of PI-IBS.
Methods
C57L/B6 mice, which have been infected by 500 Trichinella larvae for 56 days, were used to PI-IBS model mice, the levels of the intestinal mRNAs and proteins of c-kit, interleukin-10 (IL-10), and IL-17 were measured by RTPCR and western blotting or ELISA. The locations of c-kit were detected using immunohistochemical staining.
Results
Levels of the c-kit protein and mRNA were up-regulated in the whole PI-IBS mice intestine. Compared to the control bowel segments, IL-17 levels were significantly higher in the duodenum and ileum, and IL-10 levels were lower in jejunum, ileum, and colon. By immunohistochemical staining, the increased signals of the c-kit were mainly detected in the submucosa.. View More»
DOI:
10.4172/2165-8048.1000253