Journal of Pharmacological Reports
Open Access
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+44-20-4587-4809
Chakra A
India
Research Article
Bioanalytical Method Development and Validation of Doxercalciferol and 1α, 25-Dihydroxy Vitamin D2 by High Performance Liquid Chromatography Tandem Mass Spectrometry Detection: Application to Pharmacokinetic Study
Author(s): Thennati R, Shahi PK, Chakra A, Patel H, Shah V and Bhokari AThennati R, Shahi PK, Chakra A, Patel H, Shah V and Bhokari A
The systemic circulating levels of Doxercalciferol and its active metabolite form were in the pg/mL range which represents a significant bioanalytical challenge for therapeutic monitoring. Hence Liquid chromatography with tandem mass spectrometry (LC–MS/MS) with derivatization technique was considered the most appropriate standard for the selective and sensitive determination of this molecule in biological matrices and a sensitive, selective, precise and well accurate liquid chromatography tandem mass spectro-metric (LC–MS/MS) assay method was developed for simultaneous determination of Doxercalciferol and its metabolite in human plasma at pg/ml lower limit of quantitation and in order to improve the accuracy and reliability of assay method for determination of parent compound, and metabolite, a derivatization procedure was adopted. Extraction procedure was optimized with .. View More»