Journal of Medical & Surgical Pathology
Open Access
ISSN: 2472-4971
ISSN: 2472-4971
Amalia Tri Utami
Medical Program Study,
Malang
Indonesia
Research Article
Study of Antimicrobial Activity of Black Cumin Seeds (Nigella sativa L.) Against Salmonella typhi In Vitro
Author(s): Amalia Tri Utami, Bogi Pratomo and NoorhamdaniAmalia Tri Utami, Bogi Pratomo and Noorhamdani
Objective: To determine the effectiveness of extracts of black cumin seeds (Nigella sativa L.) as an antimicrobial against Salmonella typhi in vitro. And can determine minimum inhibitory concentration (MIC) and Minimal bactericidal concentration (MBC) from extracts of black cumin seeds (Nigella sativa L.) against Salmonella typhi.
Design: This experimental study used post-test only control group design with four time repetition. Step one was cultivating bacteria in liquid medium with various concentration of extract, that was 40%, 42.5%, 45%, 47.5%, 50% with two control, extract control and bacterial control.
Results: The MIC (Minimal Inhibition Concentration) is 45% concentration of extract. Step two was plating in NAP (Nutrient Agar Plate) medium. The MBC (Minimal Bactericidal Concentration) is 47.5% concentration of e.. View More»
DOI:
10.4172/2472-4971.1000127