Journal of Agricultural Science and Food Research

Journal of Agricultural Science and Food Research
Open Access

ISSN: 2593-9173

+44 1223 790975


Phosphate Solubilizing Fungi Isolated and Characterized from Teff Rhizosphere Soil Collected from North Showa and Gojam, Ethiopia

Birhanu Gizaw, Zerihun Tsegay, Genene Tefera, Endegena Aynalem, Misganaw Wassie and Endeshaw Abatneh

Phosphorus (P) is one of the major bio elements limiting agricultural production. Phosphate solubilizing fungi play a noteworthy role in increasing the bioavailability of soil phosphates for plants. The present study was aimed at isolating and characterizing phosphate solubilizing fungi from teff rhizosphere soil. Fungi were identified using lactophenol cotton blue staining confirmation and Biolog micro station. Fungi isolates were screened and transferred to Biolog universal yeast agar media. Pure yeast cells and filamentous fungi were suspended in sterile water and filamentous fungi (FF) inoculum fluid at 49 ± 2 and 75 ± 2 turbidity measured by Biolog turbidimeter respectively. 100 μ-L transferred from each suspension into 96 wells of the biolog yeast micro Plate and filamentous fungi microplate tagged with different carbon source and incubated at 26°C for 24 to 72 h and read by the micro station reader at a single wavelength of 590 nm, results were recorded and processed for identification by micro log3 software ver. 4.20.05. Biolog micro station produce 24 fungi read results. Filamentous fungi ≤ 0.5 similarity index (62.5%), yeast ≥ 0.5 similarity index (25%), yeast ≤ 0.5 similarity index (12.5%). The identified fungi were evaluated for phosphate solubilization by the pikovskaya’s agar (PVK) selective media. Seven species were positive in phosphate solubilizing ability. Trichosporon beigelii B, Phichia norvegensis, Cryptococcus albidus var aerius, Candida etchellsii, Cryptococcus albidus var albidus, Rhodotrula aurantiaca A, Rhodotorula aurantiaca B Cryptococcus luteolus, Cryptococcus albidus var diffluens, Neosartorya fisheri var. Fischeri, Cryptococcus terreus A, Candida montana, Penicillium purpurogenum var. Rubrisclerotium, Yeast isolate GTRWS18, GTS9B, GTS7C. At 15 days’ incubation, the solubilizing index ranges 1.2-5.3. The Trichosporon beigelii B, Phichia norvegensis, Cryptococcus albidus var aerius were superior in phosphate solubilization with 5.3, 3.35, 3.2 solubilizing index respectively. Therefore, these species can be candidated and exploited after further evaluation as bio fertilizers for teff productivity.