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A detailed understanding of the laws and processes that determine the ecosystem dynamics is essential in order to develop well-adjusted forestry management plans in light of climate change. This includes a good understanding of natural regeneration processes since natural regeneration can become an important component of stand resilience. Moreover, climatic changes may reduce the success of natural regeneration and hence require adjustments to silvicultural practices. This work analyzes the effect of two different soil treatments and different stand densities on the initial Pinus nigra Arn. ssp salzmanni seedlings growth at six testing sites located in the Serranía de Cuenca (Spain). Preliminary results showed that soil preparation does not affect initial seedlings growth since differences between soil preparation techniques were always not significant (P>0.05). This work also showed that Spanish black pine initial seedlings growth tended to be higher at the most common and favourable habitats than in the marginal populations. Further studies are necessary to better understand the specific influences across sites and under changing climatic conditions.