Transcriptomics: Open Access
Open Access

Abstract

Advance Gene therapeutics (Oreochromis mossambicus )

M. Sangeetha

The paper has been focussed information about the effect of tanneryeffluent on the structural and functional group changes inthe biomolecules constituting the structural architecture of themuscle and gill tissues of Oreochromis mossambicus fingerlings.Control and tannery treated fishes were given normal feed. Theexposure and period were 45 days. A range of 7 concentrations(50, 40, 30, 25, 20, 15, 10 % / L of water) were selected for lethaldose studies. The mortality in each concentration were noted for24, 48, 72 and 96 hours exposure. The LC50 value (96 hour) wasdetermined for tannery effluent exposed fingerlings of Oreochromismossambicus, which was found to be 20%. The fishes weremaintained at 50% of this concentration (10% of the tanneryeffluent). The presence of structural architecture and functionalgroup changes was confirmed by Fourier Transform Infrared(FTIR ) Spectroscopy. The FT-IR spectra of muscle and gill tissuesare composed of several bands of various functional groups havinga place with lipids, proteins and carbohydrates. The vibrationalassignment of FT-IR spectra are observed frequencies in theregion 4000–400 cm–1 along with their vibrational assignment.The ratio of the intensities of the amide bands (I 1541 / I 1652)decreases from 0.93 for control to 0.89 for tannery intoxicatedtissues during acute exposure.The I 2958 / I 2858 ratio for controland treated for gill tissues are 1.64 and 1.39. The ratio I1538 /I3290 for control, and tannery treated gill tissues are 1.81 and1.48 respectively. The ratio of the intensities of the amide bands(I1538/I1653) decreases from 0.83 for control to 0.72 for tanneryintoxicated tissues during acute exposure.

Published Date: 2020-10-11; Received Date: 2020-10-06