Will peripheral blood be the preferable source for mesenchymal st | 24724
Journal of Cell Science & Therapy

Journal of Cell Science & Therapy
Open Access

ISSN: 2157-7013

Will peripheral blood be the preferable source for mesenchymal stem cells?

4th World Congress on Cell Science & Stem Cell Research

June 24-26, 2014 Valencia Conference Centre, Valencia, Spain

Ahmed Lotfy, Yasser M El-Sherbiny, Richard Cuthbert, Elena Jones and Ahmed Badawy

Accepted Abstracts: J Cell Sci Ther

Abstract :

Background: Mesenchymal stromal cells (MSCs) possess self-renewal and multi-lineage differentiation potential this makes MSCs an attractive prospect as cellular therapeutic agents for regenerative medicine. Although adult bone marrow (BM) is the main sources for clinical use, it?s harvesting requires invasive procedures. Therefore, alternative sources are always required such as peripheral blood (PB). Objective: To compare PB-MSCs and BM-MSCs as regards their biological characteristics. Material and methods: PB-MSCs and BM-MSCs were isolated from 4 weeks oldBALB/c mice by density gradient centrifugation and then cultured in DMEM + 10% fetal bovine serum until passage four. Morphological features, proliferation, cell surface marker expression and trilineage differentiation potential were assessed for both PB-MSCs and BM-MSCs. Results: There were no significant difference in morphological features and proliferation capabilityas measured byXTT assays. Both PB-MSCs and BM-MSCs have broadly similar cell surface marker expressionbut PB-MSCs have positive expression of CD146 and CD140b. Both PB-MSCs and BM-MSCs are capable of trilineage differentiation. BM-MSCs have greater capacity for osteogenic and chondrogenic differentiation, whereas PB-MSCs have greater capacity for adipogenic differentiation. Conclusion: Although PB-MSCs and BM-MSCs have some differences; they have very similar biological characteristics. Peripheral blood is a promising easy obtained source for MSCs in mice