Vitamin E-based glycoside amphiphiles for membrane protein struct | 44447
Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

+44 1223 790975

Vitamin E-based glycoside amphiphiles for membrane protein structural studies

14th International Conference on Structural Biology

September 24-26, 2018 | Berlin, Germany

Lubna Ghani, Muhammad Ehsan and Pil Seok Chae

Hanyang University, South Korea

Posters & Accepted Abstracts: J Proteomics Bioinform

Abstract :

Membrane proteins play crucial roles in regulating cellular functions at the interface between cells and their environments or on the surface of organelles. The major bottleneck for their structure study arises from their instability outside the native environments. Amphipathic agents called detergents, form micelles which mimic the lipid environment and are widely used for protein structural and functional studies. However, conventional detergents such as DDM and OG have limitations in maintaining the solubility and stability of membrane proteins in an aqueous solution. In this study, we introduced a novel class of amphiphiles consisting of vitamin E and saccharide units as the hydrophobic and hydrophilic groups, respectively. These agents showed remarkable efficacy toward stabilization and visualization of membrane protein complex, allowing us to clearly visualize a challenging membrane protein complex using electron microscopy.

Biography :