Gynecology & Obstetrics
Open Access
ISSN: 2161-0932
ISSN: 2161-0932
Feriha Fatima, Ali Mohammad Waryah and Faiza Shabbir Ahmed
Liaquat University of Medical and Health Sciences, Pakistan
Posters & Accepted Abstracts: Gynecol Obstet
Background: Vascular endothelial growth factor (VEGF) C936T polymorphism is located at 3�?´-untranslated region (3�?´-UTR) of VEGF gene resulting alteration from C to T nucleotide. VEGF plays an important role in angiogenesis, vasculogenesis and endothelial cell migration. VEGF C936T polymorphism has been studied in relation with various gynecological and obstetric disorders including preeclampsia. Present study aimed to find out genotypes of VEGF C936T and serum levels of VEGF in preeclamptic women in comparison with normal pregnant women. Methods: This case control study was carried out at Liaquat University of Medical and Health Sciences (LUMHS) Jamshoro. Forty preeclamptic women and 40 matched gestational age normal pregnant women were selected. VEGF C936T polymorphism genotyping was carried out by tetra-primer ARMSâ�?�?PCR method whereas serum VEGF levels were determined by ELISA. Results: The homozygous CC genotype was found in 82.5% of cases and 77.5% of control group. The heterozygous CT genotype distribution was 17.5% among cases and 22.5% among controls [p=0.57; odds ratio (OR) =1.36, range: 0.45â�?�?4.12]. Serum VEGF levels were significantly higher among cases as compared to controls (p<0.05). Conclusion: This is the first study in Pakistan on association of VEGF C936T polymorphism and serum VEGF levels in preeclampsia. We did not find significant association of VEGF C936T polymorphism in development of preeclampsia in our population. However, raised serum VEGF levels in preeclampsia may suggest imbalance in angiogenic and anti- angiogenic factors in disorder and its potential as future biomarker in diagnosis and prediction of preeclampsia. ferihafatima@yahoo.com