Journal of Cell Science & Therapy
Open Access

Use of zebrafish embryos to elucidate and target disease mechanisms in vivo

International Conference on Emerging Cell Therapies

October 1-3, 2012 DoubleTree by Hilton Chicago-North Shore, USA

Jan de Sonneville

Scientific Tracks Abstracts: J Cell Sci Ther

Abstract :

A whole organism high-throughput screening system will be introduced used to study the progression of tuberculosis. Our system is capable of screening for disease progression via robotic yolk injection of early embryos and visual flow screening of late-stage larvae. Recently, injection of panels of poorly- versus highly malignant cell lines derived from breast, colorectal, and prostate cancer into zebrafish embryos have shown to correlate to long-term rodent xenograft models and mouse models. Currently, we are studying how such an automated quantitative whole animal assay can serve as a first-line in vivo screening step in the anti-cancer drug target discovery pipeline

Biography :

Jan de Sonneville done Bachelors in Electrical Engineering, Delft University of Technology (TUDelft), Master in NanoScience (Applied Physics), given as joint program by TUDelft and Leiden University, the Netherlands (2006). PhD on the development of four novel research methods for Cell Biology, thesis title: ?Reinventing microinjection, new microfluidic methods for cell biology? (2011). Founded Life Science Methods BV to sell Automated Microinjection Systems for high throughput screening using cell spheroids and zebrafish embryos (201 1).