Advancements in Genetic Engineering
Open Access

Two types of human artificial chromosome vectors

International Conference on Genetic Engineering & Genetically Modified Organisms

August 12-13, 2013 DoubleTree by Hilton, Raleigh, NC, USA

Yuichi Iida, Yasuhiro Kazuki and Mitsuo Oshimura

Posters: Adv Genet Eng

Abstract :

Conventional gene delivery vectors such as viral vectors, plasmid, bacterial artificial chromosome (BAC) might be associated with transgene-silencing because of position effect. Human artificial chromosome (HAC) vector is considered as a promising system for gene delivery and express. The HAC is produced by top-down and bottom-up approaches. Both HAC can be episomally maintained in their nuclei and segregate normally. The top-down approach can generate linear HAC ranging in approximately 3~4 Mb that retain natural centromeres and are mitotically stable in human and mouse cells. Moreover, we have succeeded in making trans-chromosomic-mice containing a HAC. It can be maintained in vivo and can produce mouse model. The bottom-up approach of HAC generation was initially developed in human fibrosarcoma HT1080 cells by introducing synthetic alphoid DNA cloned into a circular BAC vector. The tetO-HAC, generated by bottom-up approach, can be eliminated from mammalian cells such as CHO and HT1080 cells. The tetO-HAC containing human chromosome 17 alphoid DNA and tet-operater sequences in its centromeric regions resulted in controlling the centromere function. We have demonstrated that the tetO-HAC can be eliminated from nuclei in human HT1080 remarkably. The combination of using two types of HAC would be a powerful tool for cell reprogramming and gene collection of genetic disorders.

Biography :

Yuichi Iida has completed his masters degree at the age of 23 years from Tottori University, and is currently a graduate student studying for his doctoral thesis. He has published 5 papers in reputed journals