Journal of Glycobiology
Open Access

Tracking cancer progression through monitoring the dynamic serum phospho-proteome

Glycobiology World Congress

August 10-12, 2015 Philadelphia, USA

Achuth Padmanabhan

Posters-Accepted Abstracts: J Glycobiol

Abstract :

Serum proteome has been shown to reflect pathological changes in tissues. Changes in serum proteomic pattern during
carcinogenesis have been exploited successfully to serve as diagnostic and prognostic markers. Protein Kinase A catalytic
subunit �?± has been shown to be secreted into the serum as ecto-kinase and the ecto-PKA level is significantly higher in the serum
of prostate cancer patients. Here, I describe the utility of the Reverse In-gel Kinase Assay coupled with LC-MS/MS to profile
potential serum substrates of PKA and in following their phosphorylation status during disease progression. The sensitivity
and linearity of this assay will help identify potential biomarkers which can be used to track the progression of prostate cancer.
This strategy can be adapted and extended to track the progression of other cancers and pathological conditions.