Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
+44 1478 350008
ISSN: 2161-0495
+44 1478 350008
Ray Conrad E Hernandez, Jezreel Rollan M Javier, Alec Jacob S Lim, Darryl Van Q Pensotes, Edrick Glenn C Ramoran, Renz Kenneth G Cadiang and Rebecca G David
University of Santo Tomas, Philippines
Posters & Accepted Abstracts: J Clinic Toxicol
In previous studies, Vernonia cinerea (Linn.) Less. (Asteraceae) was proven for its medicinal use as an anti-inflammatory, diuretic, hepatoprotective, and nephroprotective. In order to establish the safe use of the plant as a potential drug, ethanolic leaf extract of V. cinerea (Linn.) Less was evaluated for its angiogenicity, genotoxicity and mutagenicity. Angiogenicity was determined by Chorioallantoic Membrane (CAM) assay using ten-day-old duck eggs. Sterile filter discs containing 1 g/mL of the extract or plain normal saline solution were placed on the surface of the CAM after exposing the membrane by incision. Newly formed blood vessels were counted using AngioQuant software. Genotoxicity was identified in vitro using SOSChromotest kit with an extract concentration of 1000 mcg/mL in 14 two-fold dilutions. The test utilized a genetically modified strain of Escherichia coli to determine the cell��?s response to genetic damage. The degree of DNA damage was determined through different color densities. Deep blue color indicates genotoxicity. Mutagenicity was determined in vitro using Muta- Chromo Plate test kit which employs a mutant strain of Salmonella typhimurium, carrying mutation(s) in the coding for histidine biosynthesis which when exposed to a mutagen, undergoes reverse mutation. The number of wells were observed for a change in purple to yellow color and provided the data for analysis. Based on the results, there is sufficient evidence to suggest that the ethanolic leaf extract of Vernonia cinerea (Linn.) Less (Asteraceae) exhibits no angiogenic and genotoxic properties, but exhibits mutagenic properties.
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