Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Tiziana Bonaldi
European Institute of Oncology, Italy
Posters & Accepted Abstracts: J Proteomics Bioinform
Chromatin is a highly dynamic, well-organized and yet ill-defined protein-DNA-RNA structure that controls various DNAdependent processes. A large number of site-specific post-translational modifications of histones (hPTMs) contribute to the maintenance and modulation of chromatin plasticity, gene activation, DNA replication and repair and a variety of other biological processes and disease states. The observation of the diversity, frequency and co-occurrence of histone modifications at distinct genomic loci led to the notion that these marks create a molecular barcode, read by effector proteins that translate it into a specific transcriptional state, or process, on the underlying DNA. However, the molecular details of its working mechanisms are only partially characterized. More recently, various technological progresses have enabled the detection of these PTMs on an increasing number of non-histone proteins, involved in a variety of biological processes. Recent achievements made Mass Spectrometry (MS) and quantitative proteomics excellent tools to help understanding how histone and nonhistonic PTMs mediate the structural-functional state of chromatin. My team contributed to the field by setting-up distinct MS-proteomics strategies, combined with various biochemical methods of enrichment of chromatin and extra-chromatin proteins, to investigate chromatin plasticity and nuclear dynamics governed by post-translational modifications. The talk will offer an overview of the MS-proteomics strategies developed to gain insights into chromatin biology, with emphasis on: THe proteomic dissection of chromatin regulatory regions; the hPTMs-analysis of clinical specimens and the recent achievements on the methyl-proteome profiling and its impact in DDR and miRNA biogenesis
Email: tiziana.bonaldi@ieo.eu