Journal of Pharmaceutical Care & Health Systems
Open Access
ISSN: 2376-0419
+44 1300 500008
ISSN: 2376-0419
+44 1300 500008
Rehab F Abdel-Rahman1, Hasan Yusufoglu2, Gamal A Soliman2, Irem Tatli-Cankaya3, Saleh I Alqasoumi4, Serap Arabci Anul4 and Galip Akaydin3
1National Research Centre, Egypt 2Salman bin Abdulaziz University, KSA 3Hacettepe University, Turkey 4King Saud University, KSA
Posters-Accepted Abstracts: J Pharma Care Health Sys
Thioacetamide (TAA) administration is an established technique for generating rat model of liver damage. The aim of this study was to investigate the potential hepatoprotective effect of the ethanol extracts of the aerial parts of Allium paniculatum L. (A. paniculatum) and Capparis spinosa L. (C. spinosa) in experimental liver damage induced by TAA in rats. Forty two adult male Wistar rats were divided into seven groups. Rats of the 1st (normal control) and 2nd (TAA-intoxicated control) groups received the vehicle (1 mL/kg). Animals of the 3rd group (reference) received silymarin (50 mg/kg). The 4th to the 7th groups were treated with A. paniculatum and C. spinosa extracts (200 and 400 mg/kg, respectively). Rats were administered the vehicle, silymarin or extracts orally for 21 days and simultaneously administered TAA (except the 1st group) at subcutaneous dose of 50 mg/kg, 1 h after the respective assigned treatments every 72 h. At the end of the experimental period, animals were sacrificed by cervical decapitation, blood samples were collected for biochemical assessment. Livers were dissected out for determination of their antioxidant status and for histopathological examination. Injection of TAA significantly elevated serum levels of ALT, AST, ALP and �?³-GT and total bilirubin in hepatotoxic group compared to normal controls. In the liver, significantly elevated level of malondialdehyde (MDA), lowered levels of reduced glutathione (GSH), catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) were observed in TAA-hepatotoxic group. Treatment of rats with A. paniculatum or C. spinosa displayed hepatoprotective effect in a dose dependent manner as evident by reduced levels of serum ALT, AST, ALP, �?³-GT, BRN and hepatic MDA concentration, as well as higher CAT, GPx, SOD activities and GSH concentration compared to TAA-hepatotoxic controls. In conclusion, A. paniculatum and C. spinosa extracts attenuate hepatotoxicity induced by TAA.
Email: rehabs2001@yahoo.com