Organic Chemistry: Current Research
Open Access

The Optical Sensing via Microscopic Single Particle Counting

24^th World Congress on Analytical and Bioanalytical Chemistry

March 10, 2022 | Webinar

Na Li

Peking University, China

Scientific Tracks Abstracts: Org Chemi: Cur Res

Abstract :

Single-particle based counting is not only a reasonable approach to achieve high sensitivity without the aid of target amplification, but also impart the method with unbiased quantification of multiple analytes. Based on dark-field microscopic imaging of gold nanoparticles and fluorescence microscopic imaging of fluorescent nanoparticles/microspheres, we have developed microscopic imaging based nanoparticle counting methods for single and multiple analytes in one test volume. Using colocalized fluorescence nanoparticle counting, we can achieve the six DNAs/miRNAs with less than 10 fM limit of detection in one test sample1, and Endonucleases/DNA Methyltransferases in one test sample2. Based on quantum dot doped nanoparticle counting, we can achieve simultaneous detection of proteins and miRNAs in one test sample3. These methods show the potential of applications in biological samples. We will continue to develop single particle counting based method for sensitive and multiplexed assays.

Biography :

Na Li has done her Ph.D. in Analytical Chemistry from Peking University, Beijing, China. He is currently working at Professor, College of Chemistry, Peking University. Her research interest is Optical sensing methods and applications. Major interest: Nanoparticle and its composite based spectroscopy (fluorescence, localized surface Plasmon resonance absorption or light-scattering) and the application in biosensing.