Gynecology & Obstetrics
Open Access
ISSN: 2161-0932
ISSN: 2161-0932
Duaa Abuarqoub
University of Petra-Jordan
Scientific Tracks Abstracts: Gynecol Obstet (Sunnyvale)
The clinical use of bioactive material in the fi eld of biomedical tissue engineering has increased recently. The objective of this study was to investigate if the in vitro culture of human dental pulp stem cells (hDPSCs) in media containing BiodentineTM (BD) infl uences their odonto/osteogenic differentiation potential. HDPSCs were extracted and characterized for their MSC expression profi le by fl ow cytometry. Then, the obtained cells were cultured in media containing BD for 3 weeks to study the impact of BD on their odonto/osteogenesis pathway compared to the positive control (Osteogenic media) and negative control (cell culture media). Odonto/osteogenic differentiation of hDPSCs treated with BD was evaluated by measuring the level of expression of odontoosteogenic markers by fl ow cytometry, ELISA and Alizarin red stain (ARS). In addition to the expression of genes that are involved in odonto/osteogenesis pathway by using Odonto/osteogenesis PCR array. Our results have indicated that hDPSCs treated with BD showed a signifi cant increase in the expression of odonto/osteogenic markers OCN, OPN and ALP which were measured by fl ow cytometry and ELISA. Additionally, a number of calcium deposits were detected by ARS. Moreover, genes related to odonto/osteogenesis pathway were upregulated compared to the control groups, specifi cally the genes that are involved in BMP signaling pathway, the activation of the extracellular matrix-related gene (ECMG) and Ca2 + signalling pathway. We conclude that BD showed a stimulatory effect on the odonto/ osteogenic capacity of hDPSCs, making BD a good candidate to be used clinically in dentine regeneration.
Dua’a Abuarqoub is an assistant professor in University of Petra-Jordan. She fi nished her PhD in “Stem Cell Biology”, from the University of Jordan. She also holds an MSc in “Applied Biosciences-Stem cell biology” and a BSCs in “Biotechnology and genetic engineering” from Jordan university of science and technology. She worked as a researcher in Cell Therapy Center -The university of Jordan. She was the head of dental stem cell research project, working on different types of dental stem cells, exploring their features and their differentiation potential toward different lineages. She was the head of fl ow cytometry core lab. She has over 25 publications about stem cells, and nanobiotechnology.