The ameliorative effect of gallic acid on mercuric chloride intox | 54597
Journal of Clinical Toxicology

Journal of Clinical Toxicology
Open Access

ISSN: 2161-0495

+44 1478 350008

The ameliorative effect of gallic acid on mercuric chloride intoxicated albino rats

7th Euro-Global Summit on Toxicology & Applied Pharmacology

October 24-26, 2016 Rome, Italy

Javad Babaei

Valiasr Hospital Research Center, Iran

Posters & Accepted Abstracts: J Clin Toxicol

Abstract :

This study was carried out to assessment the protective effect of gallic acid (GA) against mercuric chloride (HgCl2) induced oxidative stress in albino rats. 35 wistar male rats were divided into 5 groups. Group 1 received normal saline (2 ml/kg bwt, p.o.) for 7 days; group 2 received HgCl2 (0.4 mg/kg bwt, p.o.) daily for 7 days. Groups 3 and 4 received GA in doses of 50 and 200 mg/kg bwt respectively and an hour after the treatment with GA; HgCl2 was administrated for 7 consecutive days. Group 4 received only GA (200 mg/kg BW, p.o.) for 7 consecutive days. Results showed that HgCl2 significantly increased (p<0.05) serum levels of AST, ALT, ALP, BUN and creatinine (Cr) and lipid peroxidation (MDA) in liver and kidney tissue. Whereas, the activity of glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT) and the content of glutathione reductase (GSH) were significantly decreased in liver and kidney compared to control (p<0.05). Treatment with GA reduced serum levels of AST, ALT, ALP, BUN, Cr and MDA in liver and kidney tissue, while increased the activities of the antioxidant enzymes, and the levels of GSH. The liver tissue of HgCl2 treated showed the degenerated cells with mild cytoplasmic vacuolation and blebbing, binucleated cells and significant sinusoidal dilation. HgCl2 treated renal tissue exhibited tubular necrosis. It can be concluded that GA restores activity of the antioxidant enzymes and tissue markers in HgCl2-treated rats, probably by scavenging free radicals and reducing oxidative stress as an antioxidant.

Biography :