Targeting YopH: The major effector of the type III secretion syst | 31234
Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

+44 1223 790975

Targeting YopH: The major effector of the type III secretion system

6th International Conference & Expo on Proteomics

March 29-31, 2016 Atlanta, USA

Misgina B Girma, Afshan Begum, Christin Grundstrom1ers Ohman, Jonas Eriksson, Per Anders Enquist, Mikael Elofsson and Uwe H Sauer

Umea University, Sweden
University of Antwerpen, Belgium

Posters & Accepted Abstracts: J Proteomics Bioinform

Abstract :

Pathogenic Yersinia employs a Type III Secretion System; TSS3 is to inject their effectors into the host macrophages. One of the first proteins to be injected is the tyrosine phosphatase YopH. It is a modular protein containing the non-catalytic N-terminal domain (res.1-130) and a tyrosine phosphatase domain (res.190-468). Studies on YopH have confirmed that the bacteria lacking the protein are non-pathogenic. This makes YopH an important target for the development of Anti-Yersinia drugs. Therefore, a search for drug candidates was carried out in collaboration with the Laboratories for Chemical Biology, LCBU at Ume�?¥ University with the aim of finding small organic molecules which selectively inhibit YopH. We have cloned, purified and crystallized different constructs of YopH for binding studies and structures determination. We present our latest results of this on-going effort which involves different aspects of structural biology and biochemistry.

Biography :

Misgina B Girma is currently a PhD student in Protein Science at the Department of Biomedical Sciences, University of Antwerpen, Belgium. He has obtained his Master’s degree in Molecular Biology in 2014 from the University of Umea, Sweden. He was engaged in biochemical and biophysical characterization of the peptide calcitonin, a collaboration project between Umea and Lulea Universities, Sweden. He has completed another Master’s degree in 2010 in Fisheries and Aquatic Sciences from Addis Ababa University. Part of this study was published in the Journal of Limnologica. He has teaching experiences of biology and chemistry at Jijiga University and School of Americana, Ethiopia.