Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
ISSN: 2157-7013
Nadia Jahroudi
Scientific Tracks Abstracts: J Cell Sci Ther
Background : Cyclin dependent kinase like 1 (CDKL1) is a member of cell division control protein 2 (CDC2), plays an important impact on the progress. Th is study aimed to evaluate the related serine/threonine protein kinases family, and it is likely to occur in malignant tumors expression of CDKL1 in breast cancer and regulation in cancer cell growth. Methods: We investigated both the CDKL1 mRNA level in fresh biopsy tissues from 62 breast cancer patients and 20 benign tissues by real-time PCR, and CDKL1 protein in 20 paraffi n-embedded tissues from primary breast cancer patients by immunohistochemistry. Th e roles of CDKL1 in cell growth were analyzed with CDKL1 shRNA inhibitor-transfected cells. Results: CDKL1 was overexpressed in patients with breast cancer. CDKL1 provided a positive detection effi ciency of 89%, which was signifi cant higher than that of 44% by estrogen receptor and 27% by progesterone receptor (P<0.001). CDKL1 shRNA inhibitor-transfected cells exhibited obvious accumulation at G2/M phase and reduced cell growth when treated with the chemotherapeutic drugs. Conclusion: Both the CDKL1 level and its behavior in shRNA interference suggested that CDKL1 could be potentially developed as a tumor assistant marker for diagnosis and as a potential therapeutic target for breast cancer