Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Smita Panchal
Indian Institue of Toxicology and Research, India
Posters & Accepted Abstracts: J Proteomics Bioinform
In the present study, an ionic liquid based vortex assisted surfactant-enhanced emulsification microextraction (IL-VASEME) method followed by Plackett-Burmann Design (PBD) and Central Composite Design (CCD) using liquid chromatographyelectrospray mass spectrometry (LC-ESI-MS/MS) has been applied for the determination of fatty acids, triglycerides and phospholipids in serum samples of healthy and diabetic subjects. Study afforded the separation of these lipid classes in a single run with Rt of 5 min. The method was statistically optimized to reduce the extraction time. The extraction parameters further were optimized by design of experiment (DOE) approach. The ionic liquid, 1-butyl-3-methylimmidazolium hexafluorophosphate (BMIMPF6) was used as an extraction solvent, while surfactant Triton X-100 was used as an emulsifying agent. Statistical method, PBD screened the most significant factors such as ionic liquid volume, surfactant strength and pH for optimizing conditions for the separation of lipids. The screened factor values were based on the CCD, which was optimized as 45 �?¼L of ionic liquid, 7.5 pH and 1.25% of surfactant strength for extraction of lipids. The limit of detection (LOD) and limit of quantification (LOQ) were 0.012-0.034 ng/mL and 0.046-0.114 ng/mL respectively. The recovery of lipids was in the range of 90.9-114%. The intraday and interday precision in the serum sample ranged between 1.42-4.48% and 3.75-10.8% respectively. The study revealed that the IL-VASEME method was comparatively more sensitive with other conventional methods for the separation of both polar and non-polar lipids in single step.
Email: sptina6@gmail.com