Drug Designing: Open Access
Open Access
ISSN: 2169-0138
ISSN: 2169-0138
Tzong Rong Ger
Chung Yuan Christian University, Taiwan
Scientific Tracks Abstracts: Drug Des
Self-assembly of micelles occurs through the interaction of hydrophilic and hydrophobic regions of the copolymer. The goal of this study is to synthesize copolymeric micelles dextran-g-lauric acid that carries I�?ºB kinase B inhibitor, IMD-0354, to form a drug delivery system and to verify its effect on drug loading and encapsulation efficiency. Dextran was modified by lauric acid via an esterification process between the hydroxyl group of dextran and the carboxyl group of lauric acid. The hydrophilic dextran acts as a framework of dextran-g-lauric acid and lauric acid was grafted to its branches, forming an amphiphilic polymer. Fourier transform-infrared spectrometry and 1H nuclear magnetic resonance were used to confirm and calculate the grafting percentage of dextran- g-lauric acid. The critical micelle concentration (CMC) values were obtained using ultraviolet- visible spectrophotometry and particle size was measured using dynamic light scattering and transmission electron microscopy (TEM). The effect of drug-loaded micelles was verified using cell viability and immunofluorescence staining. TEM images indicated that the self-assembled micelles are spherical in shape. The drug loading percentage of micelles was 0.76% to 4.67% and the encapsulation efficiency was 15.4% to 93.75%. Regulating the hydrophilic and hydrophobic fragments of dextran-g-lauric acid changed the particle size, grafting rate, CMC, drug loading, and encapsulation efficiency of the polymer. IMD-0354-loaded micelles showed higher apoptosis expression in melanoma than free IMD-0354. This shows that controlled interactions of hydrophilic and hydrophobic regions on dextran-g-lauric acid may have profound effects on drug delivery for cancer treatment.
Tzong Rong Ger has received his PhD degree in Power Mechanical Engineering from National Tsing Hua University of Taiwan in 2013. He is an Assistant Professor in the Department of Biomedical Engineering, Chung Yuan Christian University of Taiwan. He has published more than 25 papers in reputed journals. His research interests include functional polymers and magnetic biomaterials for biomedical and cell engineering applications.
Email: sunbow@cycu.org.tw