Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
+44 1300 500008
ISSN: 2157-7013
+44 1300 500008
Moattari M, Kouchesfahani H, Kaka G H R and Sadraie H
Kharazmi University, Iran
Baqiyatallah University of Medical Sciences, Iran
Posters & Accepted Abstracts: J Cell Sci Ther
Purpose: The aim of this study was to determine the effects of chitosan/Polyethylene oxide scaffolds and human mesenchymal stem cells from Wharton�s jelly on regeneration of the sciatic nerve injury in Wistar rats. Methods: In this research 42 male adult rats weighting 200-250 g were divided into six groups� including; Normal group: Healthy rats without any injuries. In Control group: Sciatic nerve was cut in the middle region of thigh and epineuria were sutured. In Sham group: Culture medium was injected on the sciatic nerve damaged. In Cs/PEO group: Scaffold with a thin Cs/PEO was used around the sciatic nerve damaged. In cell group: 50000WJ-MSCs injected in the site of nerve damaged. In Cs/PEO with cell group: Scaffold with a thin Cs/PEO and 50000WJ-MSCs were used around the sciatic nerve. After 8 weeks, the rate of recovery was studied by electroctrophysiological assessments. Results: At the end of the eight week, the results of latency and amplitude of electrophysiology showed that Cs/PEO and hWJ-MSCs group was significantly better than control, sham, Cs and hWJ-MSCs groups (P<0.05). Conclusion: The results suggested that Cs/PEO had potential capability of regeneration of sciatic nerve injury.
Email: mhr.moattari@gmail.com