Stem cell in nanotoxicity testing | 32393
Journal of Pharmaceutical Care & Health Systems

Journal of Pharmaceutical Care & Health Systems
Open Access

ISSN: 2376-0419

+44 1300 500008

Stem cell in nanotoxicity testing

6th Asia-Pacific Pharma Congress

July 11-13, 2016 Kuala Lumpur, Malaysia

Yiling Hong

Western University of Health Sciences, USA

Scientific Tracks Abstracts: J Pharma Care Health Sys

Abstract :

Silver nanoparticles (AgNPs) are used extensively as antimicrobial agents in cosmetics, food products and various medical tools, but little is known about their potential toxic effects. Our aims are to determine the effects of AgNPs embryonic stem cell selfrenewal and differentiation, and use stem cell as cellular model for nanotoxicity testing. Our results indicated that AgNPs increased free radical productions and induced cell cycle arrest to stem cell via activation of p53, dephosphorylation of Rb proteins, and altered stem cell factors Oct4 expression. Furthermore we assessed neurotoxicity of AgNPs in human embryonic stem cell (hESC)- derived glutamatergic neurons (hGNs). Studies showed that citrate-coated AgNPs (AgSCs) with a zeta potential of -48 mV induced severe neurotoxicity, damaged neurite extensions and outgrowths and significantly reduced the expression of the neuronal marker MAP2, the post-synaptic density protein PSD95 and the vesicular glutamate transporter 1 at concentrations as low as 0.1 �?¼g/ml. In contrast, polyvinlypyrrolidone (PVP)-coated AgNPs (AgSPs) with a zeta potential of -37 mV only exhibited neurotoxicity at a higher concentration (5 �?¼g per ml). Therefore, our results indicated that proper coating and lower dosages of AgNPs could limit or reduce toxicity. Stem cell is an excellent cellular model to study the toxicity associated with nanoparticle exposure and the pharmaceutical drug screening.

Biography :

Yiling Hong is an Associate Professor at Western University of Health Sciences. She has received her PhD degree from University of Kentucky in 1997. She had in depth training in molecular biology and stem cell biology. As Principal Investigator of the National Institute of Health grant, her research interest is focus on stem cell differentiation and determination of cytotoxicity and genotoxocity of manufactured nanoparticle in stem cells. She has published over 35 papers.