Current Synthetic and Systems Biology
Open Access
ISSN: 2332-0737
+44-77-2385-9429
ISSN: 2332-0737
+44-77-2385-9429
Marghali Sonia, FadhlaouiImen, TouatiAsma, Zitouna Nadia, Ben RhoumaHela and Trifi-Farah Neila
Universit�?© de Tunis El Manar, Tunisia
Posters-Accepted Abstracts: Curr Synthetic Sys Biol
The Sulla genus groups six wild species originated essentially in the western Mediterranean basin. As the forages leguminous, these species present many advantages principally their high nutritive value and their good soil protection and enrichment due to extensive rhizobium. These species are well adapted to large bioclimatic conditions varying from humid to arid areas. Among these species, we have been interested particularly to two ones. These are the allogamous and only cultivated species S. coronaria and the autogamous and spontaneous S. spinosissima.The use of molecular markers is an effective way to evaluate genetic variation because they are not affected by the environment. The chosen markers in this study are the Sequence-related amplified polymorphism (SRAP) markers. The usedSRAP primer combinations exhibited high polymorphism that was demonstrated by various indices of diversity calculated by both POPGENE version 3.2 and Genalex software version 6.5. The analysis of molecular variance (AMOVA) was used to partition the total SRAP variation into within population and between populations. Variance components and the sum of all the squared differences were calculated. Moreover, the systematics of the Sulla genus was elucidated using the standardized Jaccard��?s Distance Index. The matrix was subjected to a cluster analysis via the DARWIN Software. In conclusions, the use of these markers allowed us to estimate the genetic diversity and then to improve the adaptability of the crops in the Mediterranean soils. SRAP markers could also be anchored to virtual linkage groups and then used for gene mapping.
Email: soniamarghali@yahoo.fr