Serum proteome analysis of falciparum and vivax malaria: Search for surrogate protein markers
Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

Serum proteome analysis of falciparum and vivax malaria: Search for surrogate protein markers

2nd International Conference on Proteomics & Bioinformatics

July 2-4, 2012 Embassy Suites Las Vegas, USA

Sanjeeva Srivastava and Sandipan Ray

Scientific Tracks Abstracts: J Proteomics Bioinform

Abstract :

This study was conducted to investigate serum proteome alteration in falciparum (FM) (n = 20) and vivax malaria (VM) (n = 17) patients from a malaria endemic area of India to obtain mechanistic insight about the disease pathogenesis and host immune response using multiple proteomic techniques. The eventual goal of this study was to identify potential markers that can distinguish the malaria patients from healthy controls (HC) as well as discriminate between FM and VM with high accuracy. Our results revealed differential expression of 63 and 67 (p < 0.05) serum proteins in FM and VM, respectively, compared to HC, nearly half of which (46.2%) were commonly modulated due to both plasmodial infections. Several identified proteins were found to be involved in different vital physiological pathways, including acute phase response signaling, chemokine and cytokine signaling, complement and coagulation cascades. A sub-set of the identified proteins was used to build statistical sample class prediction models employing PLSDA and other classification methods for FM, VM and HC discrimination. For the first time we have demonstrated the discrimination between falciparum and vivax malaria patients based on protein expression in host. This study will enhance our understanding about the basis of the disease pathogenesis and host immunological responses triggered by these parasitic infections. Furthermore, some of the identified differentially expressed proteins such as haptoglobin, serum amyloid A, hemopexin, and complement C4 could further be studied as potential candidate diagnostic and/or prognostic serum biomarkers for malaria.

Biography :

Sanjeeva Srivastava is Group Leader of Proteomics Laboratory at IIT Bombay India. He obtained his Ph.D. from the University of Alberta and post-doc from Harvard Medical School. Current research in this group centers on using HT proteomics for biomarker discovery in malaria and other diseases, studying protein interactions and drug target discovery. He is recipient of several awards including National Young Scientist Award (Canada), Young Scientist Awards (India) and the Apple Research Technology Support Award (UK). He serves as Editor-in-Chief for International Journal of Genomics and Proteomics, and Associate Editor for several international journals, including Journal of Integrated OMICS.