Journal of Clinical Toxicology

Journal of Clinical Toxicology
Open Access

ISSN: 2161-0495


Serum and liver tissue hydrocarbons contents of male rats orally exposed to bonny light crude oil

4th Global Summit on Toxicology

August 24-26, 2015 Philadelphia, USA

Saviour U Ufot, Friday E Uboh, Uduak O Luke and Eyong U Eyong

Scientific Tracks Abstracts: J Clin Toxicol

Abstract :

The types and concentration of hydrocarbons accumulated in the serum and liver tissues of male rats orally exposed to bonny
light crude oil (BLCO) was assessed in this study. Twenty male albino Wistar rats (180–200 g) used in this study were distributed
into two groups (control and test groups), with ten rats each. The test animals were orally exposed to 60 mg/kg bwt of BLCO,
once daily for twenty eight days, while the control animals were given distilled water. Twenty-four hours after the last exposure,
the animals in both groups were sacrificed under chloroform anesthesia, blood and liver tissues collected for analyses. The whole
blood samples were collected by cardiac puncture, allowed to clot, and serum separated after centrifugation. The serum and digested
liver tissue samples were extracted and analyzed for hydrocarbon levels using HPLC technique. The results showed that benzene,
toluene, ethylmethylene, xylene, BTEX, pyrene, naphthalene and total polycyclic aromatic hydrocarbons (PAH) in the serum
(0.066±0.004, 0.641±0.032, 0.470±0.030, 0.112±0.009, 0.370±0.080, 3.660±0.210, 0.009±0.001 12.540±0.720ug/dl, respectively) and
liver tissues (0.063±0.003, 0.604±0.024, 0.450±0.034, 0.112±0.009, 0.365±0.080, 3.620±0.190, 0.008±0.002 and 12.680±0.630 ug/g
tissue, respectively) of rats exposed to BLCO were significantly (p<0.05) higher, compared to respectively with the concentrations
in the serum (0.020±0.001, 0.015±0.001, 0.010±0.000, 0.031±0.001, 0.010±0.000, 1.040±0.010, 0.003±0.005 and 2.270±0.120 ug/
dl, respectively) and liver tissues (0.020±0.003, 0.015±0.001, 0.012±0.002, 0.031±0.001, 0.013±0.002, 1.040±0.010, 0.002±0.001 and
2.250±0.120 ug/g tissue, respectively) of control rats. The results of this study indicated that oral exposure to BLCO may results in the
accumulation of different hydrocarbons in the blood and liver tissues of rats.

Biography :

Saviour U Ufot completed his BSc in Biochemistry and MSc in Pharmacology from University of Calabar and Ibadan respectively. He completed his PhD in Biochemistry
(Biochemical and Environmental Toxicology) from University of Calabar in 2014. He was a lecturer in the Department of Pharmacology, University of Ilorin, Nigeria from 1993
to 1998. He is presently working with Total Exploration and Production Nigeria Limited as a Health, Safety and Environment specialist. He has published over 14 papers in
reputable journals and has attended many scientific seminars and conferences.