Sequence to structure and immunological analysis of MOMP from Chl | 44461
Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

+44 1223 790975

Sequence to structure and immunological analysis of MOMP from Chlamydiae

14th International Conference on Structural Biology

September 24-26, 2018 | Berlin, Germany

Francis O Atanu

Kogi State University, Nigeria

Posters & Accepted Abstracts: J Proteomics Bioinform

Abstract :

The major outer membrane protein (MOMP) is the most abundant (60% by weight) protein in the cell membrane of the Chlamydiae family. Its cellular localization makes it important for survival, host cell adhesion, invasion and other pathological schemes of Chlamydiae. MOMP has been reported to possess antibody neutralizing properties as well as provoking unique inflammatory immune response. This protein conserved in all species of Chlamydiae is a vaccine target against human and livestock diseases. This report focuses on bioinformatics and wet laboratory approaches utilized for the analysis of this 40 kDa, 389 aa protein. Bioinformatics analysis revealed that MOMP is a β-barrel protein with surface exposed peptide epitopes. Further bioinformatics using the SYBYL-X flexible docking protocol shows that the peptides formed stable complexes with MHC class II and surface exposed aliphatic side chains that may be accessible to T-cell receptors. In fact, other research groups have shown that these peptides have anti-inflammatory effect in an animal model of atherosclerosis. MOMP was effectively cloned, expressed and purified for structural studies. Analysis of MOMP by circular dichroism revealed that MOMP is a β-sheet rich protein which proved to be more thermostable in the presence of fatty acids and intermediates of the citric acid cycle. Finally, a low-resolution structure 4 �? for MOMP has been obtained by molecular replacement based on FadL of E. coli. The findings from this work opens new frontier for the development of drugs and vaccines that target MOMP.

Biography :