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Dmytro O. Minchenko, Oleksandr P. Yavorovsky, Yurij O. Paustovskyi and Oleksandr H. Minchenko
Scientific Tracks Abstracts: J Clinic Toxicol
M ethyl tertial butyl ether (MTBE) is one of a number of oxygenated compounds which are available for use in gasoline to reduce vehicle exhaust emissions, reduce the aromatic compound content, and avoid the use of organo-lead compounds, while maintaining high octane numbers. However, gasoline containing MTBE exhibited substantially more toxicity in humans than gasoline without this additive, because it provokes a variety of neurotoxic, allergic, and respiratory illnesses as well as cancers in rats and mice, but little is yet known about its low dose toxicity. In this study we have shown that prolonged treatment of rats with low concentration in air of methyl tertiary-butyl ether, toxic and ecologically dangerous chemical compounds, significantly affects the expression of PER1, ARNTL, CLOCK, CSNK1E, NUAK2 and PFKFB4 genes which are an important molecular component of circadian clock system and play a fundamental role in the control of metabolism at both the behavioral and molecular levels. The expression level of most of these genes could be an important sensitive marker of toxic action, on the organism, of methyl tertiary-butyl ether and possibly other ecologically dangerous chemical compounds, which suggests that more caution is needed in applications of MTBE as well as higher level of safety in the MTBE production industry. Moreover, the alternative splicing of PFKFB4 mRNA can also be considered a sensitive test for detection of toxic action of this ecologically dangerous chemical compound. Detailed molecular mechanisms, whereby MTBE changes the expression of these regulatory genes are complex and warrant further study
Dmytro O. Minchenko has studied at the Temple University in Philadelphia then continued to complete his M.D at the age of 28 years from the Bogomolets National Medical University and his PhD 1 year later from the Palladin Institute of Biochemistry. He is the Assistant Professor at the pediatrics No.1 Department of Bogomolets National Medical University as well as a Clinical Pediatrician at a children?s hospital and a Research Associate at the Palladin Institute of Biochemistry. He has published more than 160 publications in various journals and online publications in GenBank.