Ikram Ul Haq
GC University Lahore, Pakistan
Posters & Accepted Abstracts: J Chem Eng Process Technol
In the present study, pheolic content reduction of pre-treated wheat straw was carried out using various alkalis, acids or their combination for removal of phenolic compounds present in the biomass and to increase percentage saccharification eventually. Phosphoric acid used in combination with aqueous ammonia was selected as the best method for removal of total phenolic content. Optimization of various parameters such as conc. of alkali solution, conc. of an acid solution, a temperature of incubation and time of incubation was carried out to remove maximum phenolic content in biomass. The maximum reduction in phenolic content (48 mg GAE/g DW from 210 mg GAE/g DW of control sample) was observed when wheat straw was treated with 0.5% phosphoric acid and 5% ammonia at 110oC for 25 mins. The process of saccharification was then carried out using a detoxified, wheat straw sample and it was scaled up using a 20L fermenter. Various external, as well as internal parameters of saccharification, were optimized for the recovery of maximum total reducing sugars as well as percentage saccharification. Detoxified and grinded wheat straw (2%), saccharified at 80oC and 125rpm for 4 hours yielded 40.5% saccharification. Percentage saccharification was increased from 40.5% (achieved at lab scale) to 55.55% using optimized scale-up parameters.
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