Journal of Proteomics & Bioinformatics
Open Access

Salt-regulated genes or proteins in imperatacylindricarevealed by transcriptomic and 2D-DIGE proteomic analyses

2^nd International Conference on Proteomics & Bioinformatics

July 2-4, 2012 Embassy Suites Las Vegas, USA

Ing-Feng Chang, Min-Jey Tsai, Ren-JiuXu, Ting-Ying Wu and Ping Kao

Posters: J Proteomics Bioinform

Abstract :

Cogon grass (ImperatacylindricaL. Beauv. var. major), one of the top-ten weeds and a C4 plant, is widespread and distributed in Taiwan. In particular, Chuwei ecotype frommangrove in Danshui, Taipei was found to be salt tolerant. However, the salt tolerance mechanism is unknown. The present study was designed to identify salt-responsive genes and proteins in Chuwei ecotype which can be involved in salt tolerance mechanism.Total RNAs from shoot and root of Chuwei ecotype were isolated and subjected to next generation sequencing for transcriptome profiling. These transcript sequences were used to design probes for microarray analyses. A microarray analysis was performed to identify salt stress responsive genesup-regulated in response to 150 mM salt stress. Real-time PCR was introduced to confirm gene expression. Moreover, a proteomic analysis using 2D-(DIGE) was carried outto identify salt stress responsive proteins up-regulated in response to 150 mM salt stress. The up-regulated include photosystem proteins and antioxidant proteins. Taken together, results from microarray and proteomic study indicates that photosynthesis genes are up-regulated in the salt stress response in the salt-tolerant ecotype of Imperatacylindrica. Up-regulation of photosynthesis genes may serve one of the salt tolerant mechanismsinChuwei ecotype of Imperatacylindrica.