Role of heparin binding protein (HBP) azurocidin cap37 and brachi | 53493
Clinical & Experimental Cardiology

Clinical & Experimental Cardiology
Open Access

ISSN: 2155-9880

+44 1300 500008

Role of heparin binding protein (HBP) azurocidin cap37 and brachial artery reactivity as a prognostic test in critically ill septic patients

8th Global Cardiologists & Echocardiography Annual Meeting

July 18-20, 2016 Berlin, Germany

Nora Ismail Abbas, Gamal Hamed, Dalia Ragab, Amal Rizk, Nora Ismail and Talal Ibrahim

Cairo University, Egypt
Shebin Elkom Teaching Hospital, Egypt

Posters & Accepted Abstracts: J Clin Exp Cardiolog

Abstract :

Introduction: Rapid detection of the presence as well as optimized treatment of severe sepsis and septic shock is crucial for successful outcome. Heparin-binding protein (HBP), a potent inducer of increased vascular permeability, is a potentially useful biomarker for predicting outcome in severe sepsis patients. Ultrasound measurements of brachial artery reactivity in response to stagnant ischemia provide estimates of micro-vascular function and conduit artery endothelial function. We hypothesized that brachial artery reactivity and HBP levels would independently predict severe sepsis occurrence and mortality. Aim: To identify the role of both Heparin-binding protein (HBP) and brachial artery reactivity Compared to APACHE II and SOFA scores as predictors of morbidity and mortality in critically ill septic patients. Methods: An observational prospective controlled study of patients admitted in the Critical Care Department at Cairo University Egypt. Patients were classified into two groups GROUP I which included Sepsis (2 patients); Severe sepsis (20 patients) who had sepsis and target organ damage and Septic shock (16 patients) who had severe sepsis and developed inadequate tissue perfusion. Control group: GROUP II includes 10 critically ill patients who did not develop sepsis in their hospital clinical course. HBP Blood samples were collected at three time points during six days after admission. We measured brachial artery reactivity in 38 severe sepsis patients and in 10 control patients acute illness other than sepsis, Measurements were compared in severe sepsis patients versus control subjects and in severe sepsis survivors versus non-survivors. Multivariable analyses were also conducted. Results: Significant difference was detected between Survivors and Non-survivors in max SOFA score, Baseline WBCs, WBCs at 48 and 96 h as well as Baseline HBP, HBP at 48h and 96 h, where the result were in non-survivors versus survivors respectively{5.9(4.8-6.6)}ng/ml vs. {1.2(0.4-1.6)}ng/ml at baseline; {6.5(4.8-7.5)}ng/ml vs. {1.2(1.2-1.7)}ng/ml at 48 hours and lastly {6.0(4.5-6.6)}ng/ml vs. {1.12(0.8-1.4)} ng/ml at 96 hours. (ROC) curve analysis for prediction of severe sepsis, septic shock using Baseline HBP, AUC:0.982 , P<0.0001 with sensitivity 94.7% specificity 100%, cut-off level >1.9 ng/ml, (ROC) curve analysis for prediction of mortality using Baseline HBP level, (AUC)=0.99, P-value<0.0001, Sensitivity 91.6%, Specificity 100%, associated criterion >1.9ng/ml. Highly significant difference exists between survivors and Non-survivors respectively in FMD, Baseline velocity, Hyperemic velocity, Velocity difference and Post-deflation RI. The results were in non-survivors versus survivors respectively for FMD% {2.3(1.9-2.8)} % vs. {5.5(4.7-5.8)} %. Baseline velocity was cm/cardiac cycle 12 (9.7-14.3)} vs. {20(17.6-22)}. Hyperemic velocity (cm/cardiac cycle) was {20.5(18-25)} vs. {52.3(47.5-55)} and for Velocity difference (cm/ cardiac cycle {8.55(8-10)} vs. {31.75(28.3-34)} and for Post-deflation RI {0.78(0.72-0.81)} in Non-survivors vs. {0.53(0.48- 0.55)} Survivors. (ROC) curve analysis for prediction of severe sepsis/septic shock using:-FMD: (AUC) =0.97, P-value <0.0001, Sensitivity 94.7%, Specificity 100%, and associated criterionâ�?¤3.4 (%), Hyperemic velocity (AUC) =1, P-value<0.0001, Sensitivity 100%, Specificity 100%, associated criterion â�?¤39 (cm/card cycle). Conclusions: Plasma HBP levels and brachial artery reactivity were significantly different in patients with severe sepsis or septic shock compared to non-septic ICU patients, and both tests can predict morbidity and mortality in critically ill septic patients. Comparison of the receiver-operating characteristic (ROC- receiver-operator characteristic) curves for prediction of 28-mortality using the SOFA max, APACHE II score, HBP max, FMD and hyperemic velocity and there wasnâ�?�?t different between variable.

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