Journal of Proteomics & Bioinformatics
Open Access

Role of aquaporin in water channel system of Arabidopsis and its isolate expression in Brassica Napus

13^th International Conference on Structural and Molecular Biology: Techniques & Market Analysis

October 22-23, 2018 | Ottawa, Canada

Gurpreet Singh

Punjabi University, India

Scientific Tracks Abstracts: J Proteomics Bioinform

Abstract :

Statement of the Problem: Abiotic stresses cause significant crop loss throughout the world. Drought is one such abiotic stress that is affecting crop production. In order to mitigate drought, it is important to understand the physiology of plant and role of intrinsic proteins in overcoming the damage caused by draught. One such protein is aquaporin. Aquaporin is the member of PIPs, they are the member of the Plasma membrane intrinsic proteins family. Studies have shown that PIPs control water exchange between cytosolic and vacuolar compartments; they regulate cell turgor pressure which further regulates various activities in plants. Furthermore, PIPs also play roles in urea, and ammonia transport and abiotic stress response. Methodology & Theoretical Orientation: In this study, a PIP type aquaporin gene PIP1 was cloned from Arabidopsis and transformed to Brassica Napus using vector transfer by Agrobacterium tumefaciens through heat shock method. The transcripts were then examined through RT-PCR. Findings: The expression level of BnPIP1 was higher in the root than in the leaf and stem. The level of BnPIP1 transcript increased significantly when treated with 200 mm NaCl. Over-expression of BnPIP1 increased the root ratio and increased sensitivity to drought in transgenic Brassica napus plants. The present data provide a gene resource that can contribute to furthering our understanding of water channel protein and their application in plant stress tolerance.

Biography :

Gurpreet Singh is pursuing his Doctorate and has expertise in plant biology, abiotic stresses, and molecular biology. He is working on model plant species to unravel the molecular mechanism of plant physiology under drought conditions. He has been working on membrane transporters and their potential role in regulating water movements in the cell. He has worked on different techniques and computational tools throughout his research curriculum.

E-mail: gurpreetsbm@gmail.com