Rapid generation of sub-type, region-specific neurons and neural | 32384
Journal of Pharmaceutical Care & Health Systems

Journal of Pharmaceutical Care & Health Systems
Open Access

ISSN: 2376-0419

Rapid generation of sub-type, region-specific neurons and neural networks from human pluripotent stem cells for neurological disorders

6th Asia-Pacific Pharma Congress

July 11-13, 2016 Kuala Lumpur, Malaysia

Yiling Hong

Western University of Health Sciences, USA

Keynote: J Pharma Care Health Sys

Abstract :

Stem cell-based neuronal differentiation has provided a unique opportunity for regenerative medicine, disease modeling and drug discovery. Neurospheres are commonly used neuroprogenitors for neuronal differentiation but their clumping in culture has always been a challenge for neurodifferentiation. Here, we report a novel defined culture conditions and method for generating sub-type or region-specific neuronsfrom human embryonic and induced pluripotent stem cells without any genetic manipulation. Round and bright-edged neurospheres were generated in supplemented knockout serum replacement medium (SKSRM) under 10% CO2 which doubled the expression of NESTIN, PAX6 and FOXG1 genes compared to 5% CO2. Furthermore, an additional step (AdSTEP) was introduced to fragment the neurospheres and facilitate the formation of a monolayer neuroepithelial-type sheet and neural tube type rosette (NTTR) structure. The NTTR expressed higher level of PAX6, SOX2 and NESTIN genes compared to neuroectoderm-derived neuroprogenitors. Using these neuroprogenitors, different layers of cortical, pyramidal, GABAergic, glutamatergic, cholinergic neurons appeared within 27 days which is faster than traditional neurodifferentiation-protocols (42-60 days). With additional supplements, dopaminergic and purkinje neurons were also able to generate around 45 day too. Furthermore, our in vivo results indicated that fragmented neurospheres facilitated significantly better neurogenesis in severe combined immunodeficiency (SCID) mouse brains compared to the non-fragmented neurospheres. Therefore, this neurosphere-based neurodifferentiation protocol is a valuable tool for studies of neurodifferentiation, neuronal transplantation and high throughput screening assays.

Biography :

Yiling Hong is an Associate Professor at Western University of Health Sciences. She has received her PhD degree from University of Kentucky in 1997. She had in depth training in molecular biology and stem cell biology. As Principal Investigator of the National Institute of Health grant, her research interest is focus on stem cell differention and determination of cytotoxicity and genotoxocity of manufactured nanoparticle in stem cells. She has published over 40 papers.