Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Xianping Fang and Songlin Ruan
Accepted Abstracts: J Proteomics Bioinform
An understanding of the defense mechanisms against Colletotrichum is extremely important for breeding anthracnoseresistant strawberries. To characterize the cellular response to Colletotrichum infection, the differential proteomes of strawberry seedling leaves with and without Colletotrichum fragariae infection were analyzed at different time points by two-dimensional gel electrophoresis (2-DE) followed by MALDI-TOF/TOF identification. Mass spectrometry identified 49 differentially expressed proteins, including 30 upregulated and 19 down-regulated proteins. Notably, 2-DE analysis revealed that C. fragariae infection increased the expression of several well-known and novel pathogen-responsive proteins whose expression patterns tended to correlate with physiological leaf changes. Real time PCR was used to examine the transcriptional profiles between infected and uninfected strawberry leaves, and western blot analysis further confirmed the induction of beta-1, 3- glucanase and LMW heat shock protein in response to C. fragariae infection. In addition, considerable suppression of the expression of proteins involved in the Calvin cycle and glycolysis pathway was observed in the late phase of infection. The abundance changes of the identified proteins, together with their putative functions and participation in physiological reactions, produce a pathogen-responsive protein network in C. fragariae-challenged strawberry leaves. Together, these findings contribute to knowledge of pathogen resistance mechanisms, especially in non-model species.