Aline de Lima Leite, Heloisa Aparecida Pereira Barbosa, Tania Maria Cestari, Senda Charone, Janete Gualiume, Adriana Franco Paes Leme and Marília Afonso Rabelo Buzalaf.
Posters: J Proteomics Bioinform
In thepresentstudy, proteomic analysis was used as a tool to help understanding the molecular mechanisms underlying chronic fluoride toxicity in rat liver. Three groups of weanling male Wistarrats (21 days) were treated with drinking water containing 0 (control), 5 or 50 mg/L fluoride for 60 days (n=6/group). After euthanasia, liver and serum were collected. The left hepatic lobe and plasma were analyzes for fluoride using the ion-sensitive electrode, after hexamethyldisiloxane-facilitated diffusion. Data were analyzed by ANOVA and Tukey?s test, (p<0.05). The median hepatic lobe was used for proteomic analysis by 2D-PAGE associated with LC-MS/MS. Gels were digitalized using ImageScaner III (GE healthcar) and ANOVA statistical analysis was used to compare the differences in intensity of each matched protein spot. The p-values were calculated using the Image Master 2D Platinum v7.0 software (GE Healthcare). The spots presenting significant difference (p<0.05) were excised and identified by LCMS/ MS. From the differentially expressed spots, 94 proteins were satisfactorily identified (72.3%) and divided into 5 functional categories. Most of the proteins were related to ?metabolism and energy? (52%), followed by ?information pathways? (18%), ?cell processes? (12%), ?structure and cell organization? (11%) and ?transport? (7%). Based on the present results, it can be concluded that fluoride alters the expression of several hepatic proteins belonging to all the functional categories, especially those related to metabolism, and these alterations are more evident for the group treated with 50 mg/L fluoride.
Aline de Lima Leite is graduated in Biological Sciences from Universidadedo Sagrado Coração(USC), Bauru, Brazil.She has completed her M.Sc. in Oral Biology from Bauru Dental School, University of São Paulo (USP), and nowadays is Ph.D student in Evolutionary Genetics and Molecular Biology at Federal University of São Carlos (UFSCar), São Carlos, Brazil. Leitehas 11 published papers and 1 book chapter.