Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Ahkinyala Cobb-Abdullah, Caroline O Odewumi, Veera LD Badisa, Ramesh Katam, Michael Abazinge and Lekan M Latinwo.v
Posters: J Proteomics Bioinform
Cadmium is one of the toxic metals widely used in agriculture and industry. The accumulation of this metal in the environment is a major concern due to its carcinogenic effect. Many chemo-preventive agents have been used in the therapy against the toxic effect of heavy metals. Diallyl disulfide (DADS) a compound in garlic has been used as a preventative agent for various human disorders. The objective of this study was to examine the protective effect of DADS on the viability and proteomics analysis in rat liver cells (CRL 1439) against cadmium toxicity. The cells were treated with CdCl2 (150 μM) alone, DADS (150 μM) alone and CdCl2 (150 μM) co-treated with DADS (150 μM) for 6 h. The cells were pretreated with DADS for 2 h prior to CdCl2 treatment. The viability was measured by crystal violet dye and total proteins were isolated and resolved on high throughput two-dimensional electrophoresis to identify differentially expressed proteins. The result from viability showed an 80% decrease in CdCl2 treated cells in comparison to the control, while in co-treatment the viability was increased by 50% in comparison to the CdCl2 treated cells. In CdCl2 treated cells, 23 (+1.5 fold) up-regulated and 12 (-1.5 fold) down-regulated while in co-treated cells, different 23 (+1.5 fold) and 14 (-1.5 fold) proteins were up and down regulated. Our results clearly show the protective effect of DADS against cadmium toxicity and suggest that DADS can be used as a preventive agent against cadmium toxicity.