Journal of Proteomics & Bioinformatics
Open Access

Proteometabolic analysis revealed alteration in flavonoid and isoflavonoid metabolism upon ethylene and abscisic acid treatments in Glycine max leaves

Joint Event on 9^th International Conference and Expo on Proteomics and Molecular Medicine & 9^th International Conference on Bioinformatics

November 13-15, 2017 Paris, France

Sun Tae Kim, Ravi Gupta, Cheol Woo Min, Katharina Kramer, Ramesha H Jayaramaiah, Ganesh Kumar Agrawal, Randeep Rakwal, Ki-Hun Park, Yiming Wang and Iris Finkemeier

Pusan National University, South Korea
Max Planck Institute for Plant Breeding Research, Germany
Research Laboratory for Biotechnology and Biochemistry (RLABB), Nepal
GRADE Academy Private Limited, Nepal
University of Tsukuba, Japan
Hoshi University, Japan
Gyeongsang National University, South Korea

Posters & Accepted Abstracts: J Proteomics Bioinform

Abstract :

Phytohormones play a central role in plantâ�?�?s physiology. Despite the significant understanding in the hormone signaling, a deep understanding of downstream targets is missing, especially at the protein and metabolite levels. In this direction, here we used an integrated proteomics, phosphoproteomics and metabolomics approach to investigate the ethylene, ABA and combined ABA+ethylene signaling in soybean leaves. A protamine sulfate precipitation (PSP) method was employed to enrich the low-abundance proteins followed by their identification and quantification using label-free quantitative proteomics. This approach allowed the identification of 5171 unique proteins and 1182 differentially modulated in one or more treatments. Moreover, phosphoproteome analysis led to the identification of 716 class 1 phosphorylation sites (localization probability â�?¥0.75, score difference â�?¥5) belonging to 532 unique phosphoproteins. Increased phosphorylation of MPK3/6 was observed after ethylene treatment while ABA resulted in dephosphorylation of these MPKs. Functional annotation of the identified proteins showed an increased abundance of proteins related to the flavonoid and isoflavonoids biosynthesis in response to ethylene treatment and a shift in the fatty acid metabolism upon ABA treatment. HPLC analysis showed an accumulation of isoflavones (Genistin, Daidzein and Genistein) upon ethylene treatment, validating the proteomics results. Further, metabolome analysis using LC-MS/MS confirmed the accumulation of flavonoids and isoflavonoids in response to ethylene treatment and accumulation of lipids in response to ABA treatment. Taken together, our results showed potential cross-talks between ethylene and MPK-signaling and ABA and lipid signaling pathways.